PUM2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PUM2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11586-1-AP

Synonyms

KIAA0235, PUM2, PUMH2, Pumilio 2, Pumilio homolog 2, pumilio homolog 2 (Drosophila), PUML2

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Validation Data Gallery

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  WB analysis of mouse testis using 11586-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11586-1-AP (PUM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

11586-1-AP targets PUM2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PUM2 fusion protein Ag2179
• Full Name: pumilio homolog 2 (Drosophila)
• Calculated Molecular Weight: 114 kDa
• Observed Molecular Weight: 95 kDa, 140 kDa
• GenBank Accession Number: BC024218
• Gene Symbol: PUM2
• Gene ID (NCBI): 23369
• RRID: AB_2284639
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PUM2 (Human Pumilio homology protein 2), a member of the PUF protein family, binds to 3′ untranslated regions (3′ UTRs) of mRNA and to repress gene expression by controlling cytoplasmic polyadenylation and affecting mRNA translation [PMID:12459267]. When translationally dormant, PUM2, CPEB (CPE-binding protein) and Maskin form a complex on maternal cyclin B1 mRNA, precluding the formation of the active translation initiation complex [PMID:15687499]. PUM2 has been found to play a role in the mammalian nervous system, in particular in regulating morphology, arborization and excitability of neuronal dendrites [PMID:22016787]. The calculated molecular weight of PUM2 is 114 kDa, but the modified PUM2 protein is about 140 kDa.

Publications

Species	Application	Title
human	WB	Biomed Pharmacother; RNA binding protein PUM2 promotes the stemness of breast cancer cells via competitively binding to neuropilin-1 (NRP-1) mRNA with miR-376a.; Authors - Lansheng Zhang ; KD Validated