PUF60 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PUF60 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10810-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 10810-1-AP (same clone as 10810-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10810-1-AP (PUF60 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat testis using 10810-1-AP (same clone as 10810-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 10810-1-AP (PUF60 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 10810-1-AP (same clone as 10810-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10810-1-AP (PUF60 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 10810-1-AP (same clone as 10810-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10810-1-AP (PUF60 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10810-1-AP (same clone as 10810-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10810-1-AP (same clone as 10810-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10810-1-AP (same clone as 10810-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10810-1-AP (same clone as 10810-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

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Product Information

10810-1-PBS targets PUF60 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PUF60 fusion protein Ag1053
• Full Name: poly-U binding splicing factor 60KDa
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC008875
• Gene Symbol: PUF60
• Gene ID (NCBI): 22827
• RRID: AB_2173583
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Poly-U-binding factor 60(PUF60) is a splicing factor homologous to and complementary in function to U2AF, which involves in pre-mRNA splicing, a stepwise process initiated by binding. Also PUF60 involves in other nuclear processes such as apoptosis and transcription regulation. It can interact with and repress FUBP1-induced transcriptional activation via the core-TFIIH basal transcription factor. However, it has no repression activity in xeroderma pigmentosum complementation group B (XPB) cells.