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  • Featured Product
  • KD/KO Validated

PUF60 Polyclonal antibody

PUF60 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10810-1-AP

Synonyms

FBP interacting repressor, FIR, PUF60, Ro binding protein 1, RoBP1, RoBPI, Siah binding protein 1, Siah BP1, SIAHBP1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PUF60 Polyclonal antibody (10810-1-AP)

    WB analysis of HeLa using 10810-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10810-1-AP (PUF60 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using PUF60 Polyclonal antibody (10810-1-AP)

    WB analysis of rat testis using 10810-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 10810-1-AP (PUF60 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PUF60 Polyclonal antibody (10810-1-AP)

    WB analysis of mouse testis using 10810-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10810-1-AP (PUF60 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PUF60 Polyclonal antibody (10810-1-AP)

    WB analysis of HepG2 using 10810-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10810-1-AP (PUF60 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PUF60 Polyclonal antibody (10810-1-AP)

    IHC staining of human colon cancer using 10810-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PUF60 Polyclonal antibody (10810-1-AP)

    IHC staining of human breast cancer using 10810-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PUF60 Polyclonal antibody (10810-1-AP)

    IHC staining of human colon cancer using 10810-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PUF60 Polyclonal antibody (10810-1-AP)

    IHC staining of human breast cancer using 10810-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10810-1-AP targets PUF60 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PUF60 fusion protein Ag1053
Full Name poly-U binding splicing factor 60KDa
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC008875
Gene Symbol PUF60
Gene ID (NCBI) 22827
RRIDAB_2173583
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Poly-U-binding factor 60(PUF60) is a splicing factor homologous to and complementary in function to U2AF, which involves in pre-mRNA splicing, a stepwise process initiated by binding. Also PUF60 involves in other nuclear processes such as apoptosis and transcription regulation. It can interact with and repress FUBP1-induced transcriptional activation via the core-TFIIH basal transcription factor. However, it has no repression activity in xeroderma pigmentosum complementation group B (XPB) cells.

Protocols

Product Specific Protocols
WB protocol for PUF60 antibody 10810-1-APDownload protocol
IHC protocol for PUF60 antibody 10810-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Rep

USP22 regulates cell proliferation by deubiquitinating the transcriptional regulator FBP1.

Authors - Atanassov Boyko S BS
View Article
mouseWB

Cell Rep

CLOCK regulates Drp1 mRNA stability and mitochondrial homeostasis by interacting with PUF60.

Authors - Lirong Xu
  • KD Validated
View Article