Pentraxin 3 Recombinant antibody, PBS Only (Detector)

Pentraxin 3 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241393D3

Cat no : 84096-3-PBS

Synonyms

PTX3, Pentraxin-related protein PTX3, Pentraxin related protein PTX3, Pentaxin-related protein PTX3, Pentaxin related protein PTX3

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Validation Data Gallery

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  WB analysis using 84096-3-RR (same clone as 84096-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84096-3-RR (Pentraxin 3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84096-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01014-3

  Cytometric bead array standard curve of MP01014-3, PTX3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84096-4-PBS. Detection antibody: 84096-3-PBS. Standard: Eg1303. Range: 0.625-40 ng/mL.

• 

  Affinity and Kinetic Characterization of 84096-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84096-3-RR against Human Pentraxin 3 were performed. The affinity constant is 0.701 nM.

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Product Information

84096-3-PBS targets Pentraxin 3 as part of a matched antibody pair:

MP01014-3: 84096-4-PBS capture and 84096-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: pentraxin-related gene, rapidly induced by IL-1 beta
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: NM_002852.4
• Gene Symbol: Pentraxin 3/PTX3
• Gene ID (NCBI): 5806
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Pentraxin 3 (PTX3), also known as TNF-inducible gene 14 protein (TSG-14), is a member of the pentraxin superfamily which consists of evolutionarily conserved proteins characterized by a structural motif99, the pentraxin domain (PMID: 15771574). Pentraxin 3 can be produced by a variety of cell types including endothelial cells, smooth muscle cells, adipocytes, fibroblasts, mononuclear phagocytes, and dendritic cells, and its expression is induced by primary inflammatory signals, such as IL-1, TNF, and microbial moieties (PMID: 15771574). Pentraxin 3 is an acute phase glycoprotein that plays a role in regulating innate resistance to pathogens, inflammation, tissue remodeling and repair, female fertility, and cancer (PMID: 29412047). Pentraxin 3 is a biomarker for cardiovascular disease (PMID: 22347626). Increased plasma Pentraxin 3 levels have been found in patients with cardiovascular disorders (PMID: 22347626; 31057548). In WB, there're two major bands, an upper signal at 80-86 kDa and a lower signal at 42 kDa, representing Pentraxin 3 dimers and monomers, respectively (PMID: 18223257).