Securin Polyclonal antibody

• Featured Product
• KD/KO Validated

Securin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 18040-1-AP

Synonyms

EAP1, Esp1 associated protein, HPTTG, pituitary tumor transforming 1, PTTG, PTTG1, Securin, Securin / PTTG1, Tumor transforming protein 1, TUTR1

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Validation Data Gallery

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  WB analysis using 18040-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18040-1-AP (Securin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human urothelial carcinoma using 18040-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 18040-1-AP (Securin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 18040-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Securin / PTTG1 antibody (18040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U2OS using 18040-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Securin antibody (18040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Daudi cells, HepG2 cells, MDA-MB-231 cells
• Positive IHC detected in: human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

18040-1-AP targets Securin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Securin fusion protein Ag12670
• Full Name: pituitary tumor-transforming 1
• Calculated Molecular Weight: 202 aa, 22 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC101834
• Gene Symbol: Securin
• Gene ID (NCBI): 9232
• RRID: AB_2173410
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Securin, also known as the pituitary tumor transforming gene (PTTG1), was first isolated from rat pituitary tumor cells. Securin is a multi-functional protein that regulates sister chromatin segregation in mitosis, DNA repair, gene transcription, metabolism, and organ development. Securin is normally expressed in tissues with high proliferative activity, including the spleen, thymus gland, and testis, whereas it is rarely expressed in differentiated mature tissues (PMID: 22563433, PMID: 36123907). The calculated molecular weight of Securin is 22 kDa. With post-translational modification, the molecular weight of Securin will be migrated to 28 kDa.

Publications

Species	Application	Title
human	WB,IHC	Front Immunol; Identification and validation of tryptophan-related gene signatures to predict prognosis and immunotherapy response in lung adenocarcinoma reveals a critical role for PTTG1; Authors - Ziqiang Wang
mouse	IHC,IF	Cell Mol Life Sci; Microglial activation in an amyotrophic lateral sclerosis-like model caused by Ranbp2 loss and nucleocytoplasmic transport impairment in retinal ganglion neurons.; Authors - Kyoung-In Cho
human	WB	Sci Rep; APC/C(CDC20) and APC/C play pivotal roles in the process of embryonic development in Artemia sinica.; Authors - Mengchen Zhang
mouse	WB,IF	Cell Cycle; Chronic restraint stress disturbs meiotic resumption through APC/C-mediated cyclin B1 excessive degradation in mouse oocytes.; Authors - Junyan Sun
mouse	IF	Cryobiology; Melatonin promotes in vitro maturation of vitrified-warmed mouse GV oocytes potentially by modulating MAD2 protein expression of SAC component through MTRs.; Authors - Jinyu Yang
human	WB	Open Med (Wars); The Pseudogene PTTG3P Promotes Cell Migration and Invasion in Esophageal Squamous Cell Carcinoma.; Authors - Zhenhua Zhang ; KD Validated