PTRF Polyclonal antibody

PTRF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30086-1-AP

Synonyms

FKSG13, Cavin-1, Cavin 1

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Validation Data Gallery

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  WB analysis using 30086-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30086-1-AP (PTRF antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 30086-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 30086-1-AP (PTRF antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 30086-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PTRF antibody (30086-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HT-1080 cells, NIH/3T3 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:800-1:3200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30086-1-AP targets PTRF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTRF fusion protein Ag32786
• Full Name: polymerase I and transcript release factor
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC008849
• Gene Symbol: PTRF
• Gene ID (NCBI): 284119
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Polymerase I and transcript release factor(PTRF) is an essential factor in the biogenesis of caveolae, which involve in numerous process, such as signal transduction and membrane and lipid trafficking. PTRF can terminate transcription of RNA Pol I, which involves pausing of transcription by TTF1, and dissociation of the transcription complex, release the complex from the template. The calculated molecular weight of PTRF is 43 kDa, but modified PTRF is about 50-55 kDa. (PMID: 11139612 )