PTPRS Recombinant antibody

PTPRS Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241005B6

Cat no : 83833-5-RR

Synonyms

Receptor-type tyrosine-protein phosphatase S, R PTP sigma, R PTP S, PTPSIGMA, EC:3.1.3.48

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Validation Data Gallery

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  WB analysis using 83833-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83833-5-RR (rPTPσ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83833-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83833-5-RR against Human PTPRS were performed. The affinity constant is 68.9 pM.

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Tested Applications

• Positive WB detected in: A431 cells, SH-5Y5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83833-5-RR is PTPRS Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: protein tyrosine phosphatase, receptor type, S
• Calculated Molecular Weight: 217 kDa
• Observed Molecular Weight: 140 kDa, 217 kDa
• GenBank Accession Number: NM_002850
• Gene Symbol: PTPRS
• Gene ID (NCBI): 5802
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein tyrosine phosphatase receptor type S (PTPRS, also known as R-PTP-S, PTPSIGMA, and R-PTP-sigma) is an integral membrane protein. It can promote oligomerization by binding to large heparan sulfate proteoglycan structures, and interact with NTRK1/3, PPFIA1/2/3 (PMID: 25385546; 8524829; 9624153). The non-glycosylated molecular weight of PTPRS is 214 kDa, and the extracellular domain can be cleaved near the cell membrane by an endoprotease to generate a glycated 140 kDa protein. Variations and mutations of PTPRS are associated with Alzheimer's disease and cholangiocarcinoma (PMID: 38766183; 35991009).