PTPRS Polyclonal antibody

• Featured Product
• KD/KO Validated

PTPRS Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29415-1-AP

Synonyms

Receptor-type tyrosine-protein phosphatase sigma, Receptor-type tyrosine-protein phosphatase S, R PTP sigma, R PTP S, PTPSIGMA

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Validation Data Gallery

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  WB analysis using 29415-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29415-1-AP (PTPRS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 29415-1-AP

  WB result of PTPRS antibody (29415-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPRS transfected HEK-293 cells.

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  WB analysis using 29415-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29415-1-AP (PTPRS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 29415-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29415-1-AP (PTPRS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 29415-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29415-1-AP (PTPRS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A549 cells, HeLa cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29415-1-AP targets PTPRS in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTPRS fusion protein Ag30152
• Full Name: protein tyrosine phosphatase, receptor type, S
• Calculated Molecular Weight: 217 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_002850
• Gene Symbol: PTPRS
• Gene ID (NCBI): 5802
• RRID: AB_2918301
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPRS, also known as R-PTP-sigma or PTPσ, is a member of the protein tyrosine phosphatase (PTP) family (PMID: 8524829). PTPs dephosphorylate phosphotyrosyl residues in proteins that are phosphorylated by protein-tyrosine kinases (PTKs) (PMID: 20838449). PTPs are essential regulators of signal transduction pathways and play important roles in regulating many cellular processes in conjunction with PTKs (PMID: 7834742). PTPRS contains an extracellular region, a single transmembrane segment and two tandem intracellular catalytic domains, and thus represents a receptor-type PTP. Full-length PTPRS is 217 kDa and has been reported to be proteolytically cleaved into a 140-kDa N-terminal subunit containing extracellular and transmembrane domains and a C-terminal subunit (PMID: 29915291). This antibody raised against 825-1000 aa of human PTPRS detects endogenous PTPRS with an apparent molecular weight of 140 kDa.