PTPRO Polyclonal antibody

PTPRO Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 12161-1-AP

Synonyms

GLEPP1, PTP U2, PTPase U2, PTPRO, PTPU2, R PTP O

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Validation Data Gallery

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Published Applications

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 3 publications below
• CoIP: See 1 publications below

Product Information

12161-1-AP targets PTPRO in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTPRO fusion protein Ag2802
• Full Name: protein tyrosine phosphatase, receptor type, O
• Calculated Molecular Weight: 138 kDa
• GenBank Accession Number: BC035960
• Gene Symbol: PTPRO
• Gene ID (NCBI): 5800
• RRID: AB_2877831
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPRO(receptor-type tyrosine-protein phosphatase O) is also named as GLEPP1, PTPU2 and belongs to the protein-tyrosine phosphatase family. The protein is a receptor-like membrane protein tyrosine phosphatase expressed at the apical membrane of the podocyte foot processes in the kidney(PMID:21722858). The 1,159-amino acid predicted mature protein contains a large extracellular domain, a single transmembrane domain, and a single intracellular PTPase domain. Defects in PTPRO are the cause of nephrotic syndrome type 6 (NPHS6). In reducing conditions, PTPRO can form a smear from 180 to 220 kDa; In non-reducing conditions, a band appeares around 350 kDa, which likely represents the dimeric form of full-length PTPRO (PMID: 19573017).

Publications

Species	Application	Title
human, mouse	WB	Hepatology; Estrogen-sensitive PTPRO expression represses hepatocellular carcinoma progression by control of STAT3.; Authors - Hou Jiajie J
mouse	WB,IHC	J Hepatol; PTPRO plays a dual role in hepatic ischemia reperfusion injury through feedback activation of NF-κB.; Authors - Hou Jiajie J
mouse	WB	Oncotarget; PTPRO-mediated autophagy prevents hepatosteatosis and tumorigenesis.; Authors - Wenjie Zhang
human,mouse	WB	Cancer Commun (Lond); PTPRO represses colorectal cancer tumorigenesis and progression by reprogramming fatty acid metabolism.; Authors - Weixing Dai
mouse	WB	Cell Physiol Biochem; PTPRO Promotes Oxidized Low-Density Lipoprotein Induced Oxidative Stress and Cell Apoptosis through Toll-Like Receptor 4/Nuclear Factor κB Pathway.; Authors - Caihong Liang
human	WB	Front Pharmacol; Tyrosine Phosphatase PTPRO Deficiency in ERBB2-Positive Breast Cancer Contributes to Poor Prognosis and Lapatinib Resistance.; Authors - Hongmei Dong ; KD Validated