PTPRH Monoclonal antibody

• 

  WB analysis of pig spleen using 68635-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 68635-1-Ig (PTPRH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 68635-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 68635-1-Ig (PTPRH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

PTPRH Monoclonal Antibody for WB, ELISA

Cat No. 68635-1-Ig

Clone No.3C6E8

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, ELISA

Synonyms

SAP1, SAP 1, R PTP H, 3C6E8

---

Tested Applications

• Positive WB detected in: pig spleen tissue, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68635-1-Ig targets PTPRH in WB, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PTPRH fusion protein Ag17351
• Full Name: protein tyrosine phosphatase, receptor type, H
• Calculated Molecular Weight: 1115 aa, 122 kDa
• Observed Molecular Weight: 200-210 kDa
• GenBank Accession Number: BC111716
• Gene Symbol: PTPRH
• Gene ID (NCBI): 5794
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9HD43
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPRH, also known as SAP-1 (Stomach Cancer-Associated Protein-Tyrosine Phosphatase-1), is a member of the protein tyrosine phosphatase (PTP) family. These enzymes regulate cellular signaling by dephosphorylating tyrosine residues on proteins, counterbalancing kinase activity (PMID: 11278335). PTPRH is classified as a receptor-type PTP, characterized by an extracellular domain, a transmembrane region, and intracellular catalytic domains. In colorectal, gastric, and hepatocellular cancers, PTPRH is often upregulated and linked to poor prognosis (PMID: 12101188). Paradoxically, it may suppress tumorigenesis in other contexts by stabilizing cell adhesion. Overexpression in certain cancers suggests utility as a diagnostic or prognostic marker. The molecular weight of PTPRH is 122 kDa, it is reported that it can form a dimer with a molecular weight of 210 kDa (PMID:15850787).