PTPRE Polyclonal antibody

• Featured Product
• KD/KO Validated

PTPRE Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13922-1-AP

Synonyms

HPTPE, PTPE, PTPRE, R PTP EPSILON

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Validation Data Gallery

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  WB analysis of HL-60 using 13922-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13922-1-AP (PTPRE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 13922-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13922-1-AP (PTPRE Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 13922-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13922-1-AP (PTPRE Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 13922-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13922-1-AP (PTPRE Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 13922-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13922-1-AP (PTPRE Antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

• Positive WB detected in: HL-60 cells
• Positive IHC detected in: human breast cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

13922-1-AP targets PTPRE in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTPRE fusion protein Ag4932
• Full Name: protein tyrosine phosphatase, receptor type, E
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC050062
• Gene Symbol: PTPRE
• Gene ID (NCBI): 5791
• RRID: AB_2174672
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Front Oncol; miR-631 Inhibits Intrahepatic Metastasis of Hepatocellular Carcinoma by Targeting PTPRE.; Authors - Bingqing Chen ; KD Validated
human	IHC	Biomed Pharmacother; Inhibition of PTPRE suppresses tumor progression and improves sorafenib response in hepatocellular carcinoma; Authors - Renshun Dong
human	WB	Clin Epigenetics; TET2-mediated ECM1 hypomethylation promotes the neovascularization in active proliferative diabetic retinopathy; Authors - Chunyang Cai