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PTPN9 Polyclonal antibody
PTPN9 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 30282-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, mouse pancreas tissue, HCT 116 cells, MCF-7 cells, MDA-MB-468 cells, SW480 cells, rat pancreas tissue |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
30282-1-AP targets PTPN9 in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse samples.
Tested Reactivity | Human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PTPN9 fusion protein Ag31971 |
Full Name | protein tyrosine phosphatase, non-receptor type 9 |
Calculated Molecular Weight | 593 aa, 68 kDa |
Observed Molecular Weight | 68-70 kDa |
GenBank Accession Number | BC010863 |
Gene Symbol | PTPN9 |
Gene ID (NCBI) | 5780 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PTPN9, Tyrosine-protein phosphatase non-receptor type 9, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN9 is involved in the transfer of hydrophobic ligands or in functions of the Golgi apparatus (PMID: 19167335). MiR-96 inhibits PTPN9 expression and consequently promotes proliferation, migration and invasion of breast cancer cells (PMID:27857177). PTPN9 can be detected double bands (PMID: 22394684). PTPN9 was located in cytoplasm and nucleus in human breast cancer (PMID: 23418360).
Protocols
Product Specific Protocols | |
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WB protocol for PTPN9 antibody 30282-1-AP | Download protocol |
IHC protocol for PTPN9 antibody 30282-1-AP | Download protocol |
IP protocol for PTPN9 antibody 30282-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |