PTPN9 Polyclonal antibody

PTPN9 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12109-1-AP

Synonyms

MEG2, PTPase MEG2, PTPMEG2, PTPN9

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Validation Data Gallery

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  WB analysis of mouse pancreas using 12109-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of A549 using 12109-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12109-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 12109-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 12109-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 12109-1-AP (PTPN9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 12109-1-AP

  IP result of anti-PTPN9 (IP:12109-1-AP, 4ug; Detection:12109-1-AP 1:300) with HEK-293 cells lysate 1800ug.

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  IHC staining of human liver cancer using 12109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12109-1-AP (PTPN9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12109-1-AP (PTPN9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, Transfected HEK-293 cells, mouse liver tissue, mouse pancreas tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12109-1-AP targets PTPN9 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTPN9 fusion protein Ag2754
• Full Name: protein tyrosine phosphatase, non-receptor type 9
• Calculated Molecular Weight: 593 aa, 68 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC010863
• Gene Symbol: PTPN9
• Gene ID (NCBI): 5780
• RRID: AB_2877826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPN9, Tyrosine-protein phosphatase non-receptor type 9, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN9 is involved in the transfer of hydrophobic ligands or in functions of the Golgi apparatus (PMID: 19167335). MiR-96 inhibits PTPN9 expression and consequently promotes proliferation, migration and invasion of breast cancer cells (PMID:27857177). PTPN9 can be detected double bands (PMID: 22394684). PTPN9 was located in cytoplasm and nucleus in human breast cancer (PMID: 23418360).

Publications

Species	Application	Title
mouse	WB	Mol Ther Nucleic Acids; MicroRNA-126 Regulates Angiogenesis and Neurogenesis in a Mouse Model of Focal Cerebral Ischemia.; Authors - Meijie Qu