PTPN5 Polyclonal antibody

PTPN5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14515-1-AP

Synonyms

PTPN5, PTPSTEP, STEP

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Validation Data Gallery

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  WB analysis of HL-60 using 14515-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14515-1-AP (PTPN5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14515-1-AP

  IP result of anti-PTPN5 (IP:14515-1-AP, 4ug; Detection:14515-1-AP 1:1500) with mouse brain tissue lysate 1840 ug.

• 

  IHC staining of mouse spleen using 14515-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 14515-1-AP (PTPN5 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse spleen using 14515-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 14515-1-AP (PTPN5 Antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

• Positive WB detected in: HL-60 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14515-1-AP targets PTPN5 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTPN5 fusion protein Ag5974
• Full Name: protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched)
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 46-50 kDa, 61-66 kDa
• GenBank Accession Number: BC064807
• Gene Symbol: PTPN5
• Gene ID (NCBI): 84867
• RRID: AB_2173548
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Mol Neurobiol; The Weakened Interaction Between HECTD4 and GluN2B in Ischemic Stroke Promotes Calcium Overload and Brain Injury Through a Mechanism Involving the Decrease of GluN2B and MALT1 Ubiquitination; Authors - Yi-Yue Zhang