PTPN14 Monoclonal antibody

PTPN14 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1B8C8

Cat no : 67744-1-Ig

Synonyms

PEZ, PTP36, PTPN14

---

Validation Data Gallery

• 

  WB analysis using 67744-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67744-1-Ig (PTPN14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 67744-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67744-1-Ig (PTPN14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 67744-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67744-1-Ig (PTPN14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: U2OS cells, A549 cells, HEK-293 cells, Hela cells, K-562 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

67744-1-Ig targets PTPN14 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PTPN14 fusion protein Ag12679
• Full Name: protein tyrosine phosphatase, non-receptor type 14
• Calculated Molecular Weight: 1187 aa, 135 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC101754
• Gene Symbol: PTPN14
• Gene ID (NCBI): 5784
• RRID: AB_2918513
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPN14 (tyrosine-protein phosphatase non-receptor type 14) is also named as PEZ and belongs to the protein-tyrosine phosphatase family and non-receptor class subfamily. It is a multi-domain protein expressed in breast, kidney, skeletal muscle, lung, placenta, and characterized by an N-terminal FERM domain and a C-terminal PTP domain with an intervening sequence containing an acidic region and a putative SH3 domain-binding sequence (PMID: 21701840). Defects in PTPN14 are a cause of choanal atresia and lymphedema (CHATLY).

Publications

Species	Application	Title
human	WB,IF	Biomed Pharmacother; XPO1 intensifies sorafenib resistance by stabilizing acetylation of NPM1 and enhancing epithelial-mesenchymal transition in hepatocellular carcinoma; Authors - Zengbin Wang