PTPN13 Polyclonal antibody

• Featured Product
• KD/KO Validated

PTPN13 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 25944-1-AP

Synonyms

FAP 1, hPTPE1, PNP1, PTP BAS, PTP BL, PTP E1, PTP1E, PTPL1, PTPLE, PTPN13

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Validation Data Gallery

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  WB analysis using 25944-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25944-1-AP (PTPN13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 25944-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25944-1-AP (PTPN13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 25944-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25944-1-AP (PTPN13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: human breast cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 25944-1-AP is PTPN13 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTPN13 fusion protein Ag22854
• Full Name: protein tyrosine phosphatase, non-receptor type 13 (APO-1/CD95 (Fas)-associated phosphatase)
• Calculated Molecular Weight: 2485 aa, 277 kDa
• Observed Molecular Weight: 277 kDa
• GenBank Accession Number: BC140777
• Gene Symbol: PTPN13
• Gene ID (NCBI): 5783
• RRID: AB_2880304
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	PLoS One; Distinctive genes and signaling pathways associated with type 2 diabetes-related periodontitis: Preliminary study; Authors - Poliana Mendes Duarte
human	WB	Oral Dis; PMID: 32730654; Authors - Lu-Ming Wang ; KD Validated
human	WB	Thorac Cancer; Protein tyrosine phosphatase PTPL1 suppresses lung cancer through Src/ERK/YAP1 signaling; Authors - Jing Wang ; KD Validated
human	WB	Cell Death Dis; Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration; Authors - Xiaoman Zhu
human	WB,IF	Int J Mol Sci; PTPN13 Participates in the Regulation of Epithelial-Mesenchymal Transition and Platinum Sensitivity in High-Grade Serous Ovarian Carcinoma Cells; Authors - Leticia Aptecar
	WB	Cancer Med; METTL1/FOXM1 promotes lung adenocarcinoma progression and gefitinib resistance by inhibiting PTPN13 expression; Authors - Wei Peng