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PTPN12 Polyclonal antibody, PBS Only

PTPN12 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 31215-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPN12 Polyclonal antibody (31215-1-AP)

    WB analysis using 31215-1-AP (same clone as 31215-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31215-1-AP (PTPN12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31215-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PTPN12 Polyclonal antibody (31215-1-AP)

    IF Staining of A431 using 31215-1-AP (same clone as 31215-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PTPN12 antibody (31215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31215-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using PTPN12 Polyclonal antibody (31215-1-AP)

    IF Staining of U-251 using 31215-1-AP (same clone as 31215-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using PTPN12 antibody (31215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31215-1-PBS in a different storage buffer formulation.

Product Information

31215-1-PBS targets PTPN12 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPN12 fusion protein Ag35382
Full Name protein tyrosine phosphatase, non-receptor type 12
Calculated Molecular Weight88kDa
Observed Molecular Weight110-125 kDa
GenBank Accession NumberNM_002835.3
Gene Symbol PTPN12
Gene ID (NCBI) 5782
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PTPN12 (Tyrosine-protein phosphatase non-receptor type 12), also referred to as PTP-PEST (PTP-proline, glutamic acid, serine, and threonine-rich), is a cytoplasmic PTP expressed in a wide spectrum of cell types. PTPN12 has been identified as a potent tumor suppressor in human cancers and a critical regulator of cell adhesion and migration (PMID: 26391955, 29743287). PTPN12 is phosphorylated on serine and threonine residues and is rich in proline (P), glutamate (E), serine (S), and threonine (T) residues. Western blot analysis detected PTPN12 at an apparent molecular mass of 110 kDa.