PTPN12 Polyclonal antibody

PTPN12 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31215-1-AP

Synonyms

PTPG1, PTP PEST

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Validation Data Gallery

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  WB analysis using 31215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31215-1-AP (PTPN12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 31215-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PTPN12 antibody (31215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of U-251 using 31215-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using PTPN12 antibody (31215-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, HepG2 cells, MCF-7 cells
• Positive IF/ICC detected in: A431 cells, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31215-1-AP targets PTPN12 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTPN12 fusion protein Ag35382
• Full Name: protein tyrosine phosphatase, non-receptor type 12
• Calculated Molecular Weight: 88kDa
• Observed Molecular Weight: 110-125 kDa
• GenBank Accession Number: NM_002835.3
• Gene Symbol: PTPN12
• Gene ID (NCBI): 5782
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPN12 (Tyrosine-protein phosphatase non-receptor type 12), also referred to as PTP-PEST (PTP-proline, glutamic acid, serine, and threonine-rich), is a cytoplasmic PTP expressed in a wide spectrum of cell types. PTPN12 has been identified as a potent tumor suppressor in human cancers and a critical regulator of cell adhesion and migration (PMID: 26391955, 29743287). PTPN12 is phosphorylated on serine and threonine residues and is rich in proline (P), glutamate (E), serine (S), and threonine (T) residues. Western blot analysis detected PTPN12 at an apparent molecular mass of 110 kDa.