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PTPN11/SHP2 Recombinant antibody
PTPN11/SHP2 Uni-rAbTM Recombinant Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
5L23
Cat no : 82503-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, A431 cells, HEK-293 cells, A549 cells, NIH/3T3 cells, HSC-T6 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
82503-1-RR targets PTPN11/SHP2 in WB, IP, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | PTPN11/SHP2 fusion protein Ag13660 |
Full Name | protein tyrosine phosphatase, non-receptor type 11 |
Calculated Molecular Weight | 597 aa, 68 kDa |
Observed Molecular Weight | 68 kDa |
GenBank Accession Number | BC008692 |
Gene Symbol | PTPN11 |
Gene ID (NCBI) | 5781 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PTPN11(protein tyrosine phosphatase, non-receptor type 11), also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, and MGC14433, belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism(PMID:19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions(PMID:21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML), and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.
Protocols
Product Specific Protocols | |
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WB protocol for PTPN11/SHP2 antibody 82503-1-RR | Download protocol |
IHC protocol for PTPN11/SHP2 antibody 82503-1-RR | Download protocol |
IP protocol for PTPN11/SHP2 antibody 82503-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |