PTPIP51 Monoclonal antibody

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  WB analysis using 68582-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68582-1-Ig (PTPIP51 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IF Staining of HeLa using 68582-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTPIP51 antibody (68582-1-Ig, Clone: 1G11E2 ) at dilution of 1:476 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

PTPIP51 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 68582-1-Ig

Clone No.1G11E2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

FAM82A2, 1G11E2, hucep-10, hRMD-3, hRMD 3

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Tested Applications

• Positive WB detected in: LNCaP cells, HepG2 cells, HuH-7 cells, HT-29 cells, HEK-293 cells, K-562 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:238-1:952

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68582-1-Ig targets PTPIP51 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PTPIP51 fusion protein Ag15135
• Full Name: family with sequence similarity 82, member A2
• Calculated Molecular Weight: 470 aa, 52 kDa
• Observed Molecular Weight: 52-60 kDa
• GenBank Accession Number: BC008970
• Gene Symbol: PTPIP51
• Gene ID (NCBI): 55177
• RRID: AB_3085282
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96TC7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein tyrosine phosphatase-interacting protein 51 (PTPIP51),also known as RMDN3, is a mitochondrial membrane-anchored protein that interacts with ER membrane-bound VAPB. The VAPB-PTPIP51 interaction impacts on intracellular Ca2+ handling (PMID: 22131369, 28345618). PTPIP51 participates in multiple cellular processes, and dysfunction of PTPIP51 is implicated in diseases such as cancer and neurodegenerative disorders (PMID: 28345618).The known initiation sites in the Ptpip51 gene would lead to molecular protein masses of 52, 38 and 30 kDa (PMID: 18854601,19844996).