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PTPIP51 Polyclonal antibody
PTPIP51 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IHC, IF/ICC, IP, COIP, ELISA
Conjugate
Unconjugated
Cat no : 20641-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, Jurkat cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human skin tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 8 publications below |
IHC | See 2 publications below |
IF | See 7 publications below |
CoIP | See 1 publications below |
Product Information
The immunogen of 20641-1-AP is PTPIP51 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat, monkey |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PTPIP51 fusion protein Ag14708 |
Full Name | family with sequence similarity 82, member A2 |
Calculated Molecular Weight | 470 aa, 52 kDa |
Observed Molecular Weight | 52-60 kDa |
GenBank Accession Number | BC008970 |
Gene Symbol | PTPIP51 |
Gene ID (NCBI) | 55177 |
RRID | AB_10695187 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for PTPIP51 antibody 20641-1-AP | Download protocol |
IHC protocol for PTPIP51 antibody 20641-1-AP | Download protocol |
IF protocol for PTPIP51 antibody 20641-1-AP | Download protocol |
IP protocol for PTPIP51 antibody 20641-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Cell Biol MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.
| ||
Mol Cell The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation. | ||
J Cell Biol Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy. | ||
J Cell Sci The role of Mfn2 in the structure and function of endoplasmic reticulum-mitochondrial tethering in vivo. | ||
Sci Rep Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Tom (Verified Customer) (03-23-2021) | Cos7 cells fixed in 4% PFA, perm in 0.1% Triton then blocked in 1% BSA. Mitochondria also labelled with TOMM20. Cells incubated with primary antibody (1/100) for 1 hour.
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