PTP4A1 Monoclonal antibody, PBS Only

PTP4A1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C9E10

Cat no : 67584-1-PBS

Synonyms

PRL1, PTP4A1/PRL1, Protein-tyrosine phosphatase of regenerating liver 1, Protein-tyrosine phosphatase 4a1, PRL-1

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Validation Data Gallery

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  WB analysis using 67584-1-Ig (same clone as 67584-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67584-1-Ig (PTP4A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67584-1-PBS in a different storage buffer formulation.

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  WB analysis using 67584-1-Ig (same clone as 67584-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67584-1-Ig (PTP4A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67584-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67584-1-Ig (same clone as 67584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67584-1-Ig (PTP4A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67584-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67584-1-Ig (same clone as 67584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67584-1-Ig (PTP4A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67584-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67584-1-Ig (same clone as 67584-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTP4A1 antibody (67584-1-Ig, Clone: 2C9E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67584-1-PBS in a different storage buffer formulation.

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Product Information

67584-1-PBS targets PTP4A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PTP4A1 fusion protein Ag14436
• Full Name: protein tyrosine phosphatase type IVA, member 1
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC023975
• Gene Symbol: PTP4A1
• Gene ID (NCBI): 7803
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PTP4A1(protein tyrosine phosphatase type IVA, member 1), also named as PRL1, HH72, PRL-1, PTP(CAAX1), PTPCAAX1, DKFZp779M0721, belongs to the protein-tyrosine phosphatase family, which play an important role in maintaining appropriate tyrosine phosphorylation levels in the cell during development and tissue regeneration (PMID:19341304). PTP4A1 is a PTPase and that it is highly expressed in growing hepatic cells and some tumor cell lines and is located primarily in the cell nucleus, and its overexpression modulates the growth of cells (PMID:8196618). PTP4A1 can exist as dimer, trimer and higher oligomers in vivo (PMID: 15571731, 18224294).