PTP1B Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PTP1B Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11334-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of Jurkat using 11334-1-AP (same clone as 11334-1-PBS)

  WB result of PTP1B antibody (11334-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTP1B transfected Jurkat cells. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Sp2/0 using 11334-1-AP (same clone as 11334-1-PBS)

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 11334-1-AP (same clone as 11334-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11334-1-AP (same clone as 11334-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

• 

  WB analysis of BxPC-3 using 11334-1-AP (same clone as 11334-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 11334-1-AP (same clone as 11334-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 11334-1-AP (same clone as 11334-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 11334-1-AP (same clone as 11334-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11334-1-AP (same clone as 11334-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11334-1-AP (same clone as 11334-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 11334-1-AP (same clone as 11334-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 11334-1-AP (same clone as 11334-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 11334-1-AP (same clone as 11334-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

• 

  IP experiment of A431 using 11334-1-AP (same clone as 11334-1-PBS)

  IP result of anti-PTP1B (IP:11334-1-AP, 4ug; Detection:11334-1-AP 1:500) with A431 cells lysate 2400ug. This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11334-1-AP (same clone as 11334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11334-1-AP (PTP1B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

• 

  IF Staining of BxPC-3 using 11334-1-AP (same clone as 11334-1-PBS)

  Immunofluorescent analysis of BxPC-3 cells using 11334-1-AP (PTP1B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11334-1-PBS in a different storage buffer formulation.

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Product Information

11334-1-PBS targets PTP1B in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTP1B fusion protein Ag1878
• Full Name: protein tyrosine phosphatase, non-receptor type 1
• Calculated Molecular Weight: 435 aa, 50 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC015660
• Gene Symbol: PTP1B
• Gene ID (NCBI): 5770
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PTPN1 (protein tyrosine phosphatase, non-receptor type 1) is also named as PTP1B or PTP-1B, and belongs to the protein-tyrosine phosphatase family and non-receptor class 1 subfamily. PTPN1 is involved in the regulation of several important physiological pathways, which regulates both INS and leptin signaling, and interacts with the epidermal-and platelet-derived growth factor receptors (PMID: 20101100). The gene might harbor variants influencing susceptibility to INS resistance and type 2 diabetes (PMID:15919813). The molecular mass of PTPN1 is 50 kDa, but sometimes some truncated forms can be detected as 48, 46, 39, 37 kDa due to non-specific proteolytic cleavage of PTP1B in different cells (PMID: 18253097).