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  • Featured Product
  • KD/KO Validated

PTP1B Polyclonal antibody

PTP1B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11334-1-AP

Synonyms

PTPN1, PTPN 1, PTP-1B, PTP 1B, Protein-tyrosine phosphatase 1B

Validation Data Gallery

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  • Western Blot (WB) analysis of Sp2/0 cells using PTP1B Polyclonal antibody (11334-1-AP)

    WB analysis of Sp2/0 using 11334-1-AP

    Sp2/0 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PTP1B Polyclonal antibody (11334-1-AP)

    WB analysis of Jurkat using 11334-1-AP

    WB result of PTP1B antibody (11334-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTP1B transfected Jurkat cells.

  • Western Blot (WB) analysis of BxPC-3 cells using PTP1B Polyclonal antibody (11334-1-AP)

    WB analysis of BxPC-3 using 11334-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using PTP1B Polyclonal antibody (11334-1-AP)

    WB analysis of human placenta using 11334-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using PTP1B Polyclonal antibody (11334-1-AP)

    WB analysis of human testis using 11334-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using PTP1B Polyclonal antibody (11334-1-AP)

    WB analysis of HL-60 using 11334-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using PTP1B Polyclonal antibody (11334-1-AP)

    WB analysis of PC-3 using 11334-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PTP1B Polyclonal antibody (11334-1-AP)

    WB analysis using 11334-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PTP1B Polyclonal antibody (11334-1-AP)

    WB analysis using 11334-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PTP1B Polyclonal antibody (11334-1-AP)

    WB analysis of Jurkat using 11334-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using PTP1B Polyclonal antibody (11334-1-AP)

    WB analysis of THP-1 using 11334-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using PTP1B Polyclonal antibody (11334-1-AP)

    WB analysis of A431 using 11334-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PTP1B Polyclonal antibody (11334-1-AP)

    WB analysis using 11334-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using PTP1B Polyclonal antibody (11334-1-AP)

    IP experiment of A431 using 11334-1-AP

    IP result of anti-PTP1B (IP:11334-1-AP, 4ug; Detection:11334-1-AP 1:500) with A431 cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PTP1B Polyclonal antibody (11334-1-AP)

    IHC staining of human prostate cancer using 11334-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11334-1-AP (PTP1B antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using PTP1B Polyclonal antibody (11334-1-AP)

    IF Staining of BxPC-3 using 11334-1-AP

    Immunofluorescent analysis of BxPC-3 cells using 11334-1-AP (PTP1B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inSp2/0 cells, BxPC-3 cells, A431 cells, HeLa cells, Jurkat cells, THP-1 cells, mouse liver tissue, human testis tissue, HL-60 cells, human placenta tissue, C6 cells, NIH/3T3 cells, mouse placenta tissue, rat liver tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11334-1-AP targets PTP1B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTP1B fusion protein Ag1878
Full Name protein tyrosine phosphatase, non-receptor type 1
Calculated Molecular Weight 435 aa, 50 kDa
Observed Molecular Weight 45-55 kDa
GenBank Accession NumberBC015660
Gene Symbol PTP1B
Gene ID (NCBI) 5770
RRIDAB_10642566
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPN1 (protein tyrosine phosphatase, non-receptor type 1) is also named as PTP1B or PTP-1B, and belongs to the protein-tyrosine phosphatase family and non-receptor class 1 subfamily. PTPN1 is involved in the regulation of several important physiological pathways, which regulates both INS and leptin signaling, and interacts with the epidermal-and platelet-derived growth factor receptors (PMID: 20101100). The gene might harbor variants influencing susceptibility to INS resistance and type 2 diabetes (PMID:15919813). The molecular mass of PTPN1 is 50 kDa, but sometimes some truncated forms can be detected as 48, 46, 39, 37 kDa due to non-specific proteolytic cleavage of PTP1B in different cells (PMID: 18253097).

Protocols

Product Specific Protocols
WB protocol for PTP1B antibody 11334-1-APDownload protocol
IHC protocol for PTP1B antibody 11334-1-APDownload protocol
IF protocol for PTP1B antibody 11334-1-APDownload protocol
IP protocol for PTP1B antibody 11334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Ginseng Res

Ginsenoside compound K reduces ischemia/reperfusion-induced neuronal apoptosis by inhibiting PTP1B-mediated IRS1 tyrosine dephosphorylation

Authors - Jing Fu
View Article
humanWB

Aging (Albany NY)

Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells.

Authors - Changying Shi
View Article
ratWB

J Cell Physiol

Circular RNA circTET3 mediates migration of rat vascular smooth muscle cells by targeting miR-351-5p.

Authors - Qing-Ping Yao
View Article
mouseWB,IHC

FASEB J

PMID: 32100381

Authors - Ping Yang
View Article
humanWB

Front Cell Dev Biol

Tubeimoside I Inhibits Cell Proliferation and Induces a Partly Disrupted and Cytoprotective Autophagy Through Rapidly Hyperactivation of MEK1/2-ERK1/2 Cascade via Promoting PTP1B in Melanoma.

Authors - Juan Du
  • KD Validated
View Article
mouseWB

Eur J Pharmacol

Oleanolic acid derivative alleviates cardiac fibrosis through inhibiting PTP1B activity and regulating AMPK/TGF-β/Smads pathway

Authors - An-Hui Wang
View Article