PTH1R Polyclonal antibody

PTH1R Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29115-1-AP

Synonyms

parathyroid hormone 1 receptor, PFE, PTH/PTHr receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTH1R, PTHR, PTHR1

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Validation Data Gallery

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  WB analysis using 29115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29115-1-AP (PTH1R antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29115-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29115-1-AP (PTH1R antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 29115-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29115-1-AP (PTH1R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 29115-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29115-1-AP (PTH1R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 29115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29115-1-AP (PTH1R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 29115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29115-1-AP (PTH1R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse kidney tissue, HepG2 cells, mouse liver tissue, rat kidney tissue, rat liver tissue
• Positive IHC detected in: human pancreas cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29115-1-AP targets PTH1R in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTH1R fusion protein Ag30762
• Full Name: parathyroid hormone 1 receptor
• Calculated Molecular Weight: 593 aa, 66 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC110388
• Gene Symbol: PTH1R
• Gene ID (NCBI): 5745
• RRID: AB_2918237
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTH1R is a receptor for parathyroid hormone and parathyroid hormone-related peptide (PTHrP). PTH1R is a glycoprotein with seven transmembrane domains and it has an extracellular N-terminus region and an intracellular C-terminus region. The activity of PTH1R is mediated by G proteins which activate adenylyl cyclase. It has been reported that PTHrP and PTH1R are associated with the differentiation of bone and cartilage in the developing stages. PTH1R has several isoforms with 66/59 kDa and PTH1R can form a 120 kDa dimer in the non-reducing condition.