PTGR1 Polyclonal antibody

PTGR1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13374-1-AP

Synonyms

LTB4DH, PGR1, PRG 1, prostaglandin reductase 1, PTGR1, ZADH3

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Validation Data Gallery

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  WB analysis using 13374-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13374-1-AP (PTGR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13374-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13374-1-AP (PTGR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13374-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13374-1-AP (PTGR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 13374-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 13374-1-AP (PTGR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13374-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13374-1-AP (PTGR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 13374-1-AP

  IP result of anti-PTGR1 (IP:13374-1-AP, 4ug; Detection:13374-1-AP 1:500) with HepG2 cells lysate 3200ug.

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Tested Applications

• Positive WB detected in: HeLa cells, human colon tissue, human liver tissue, mouse liver tissue, HepG2 cells, rar liver tissue
• Positive IP detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

13374-1-AP targets PTGR1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTGR1 fusion protein Ag4196
• Full Name: prostaglandin reductase 1
• Calculated Molecular Weight: 329 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC035228
• Gene Symbol: PTGR1
• Gene ID (NCBI): 22949
• RRID: AB_2173213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTGR1(Prostaglandin reductase 1) is also named as LTB4DH(NADP-dependent leukotriene B4 12-hydroxydehydrogenase) and belongs to the NADP-dependent oxidoreductase L4BD family. It catalyses the conversion of leukotriene B4 into biologically less reactive 12-oxo-leukotriene B4, a key step for its inactivation and it is a key oxidation step in the process of metabolic inactivation of leukotriene B(4) in various non-leukocyte tissues(PMID:8576264).

Publications

Species	Application	Title
human	WB	J Biol Chem; Modulation of Nitro-Fatty Acid Signaling: Prostaglandin Reductase-1 is a Nitroalkene Reductase.; Authors - Vitturi Dario A DA
human	WB	Mol Cancer Ther; Conditional Dependency of LP-184 on Prostaglandin Reductase 1 is Synthetic Lethal in Pancreatic Cancers with DNA Damage Repair Deficiencies; Authors - Diana Restifo
human	WB	Int J Biol Sci; NUCB2 promotes hepatocellular carcinoma cell growth and metastasis by activating the E2F4/PTGR1 axis; Authors - Yuan Wang