PTGIS Polyclonal antibody

PTGIS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27061-1-AP

Synonyms

CYP8, CYP8A1, PGIS, Prostacyclin synthase, Prostaglandin I2 synthase, PTGI, PTGIS



Tested Applications

Positive WB detected inmouse lung tissue, mouse testis tissue, rat lung tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSaos-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27061-1-AP targets PTGIS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTGIS fusion protein Ag25817
Full Name prostaglandin I2 (prostacyclin) synthase
Calculated Molecular Weight 500 aa, 57 kDa
Observed Molecular Weight50-57 kDa
GenBank Accession NumberBC101809
Gene Symbol PTGIS
Gene ID (NCBI) 5740
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PTGIS antibody 27061-1-APDownload protocol
IHC protocol for PTGIS antibody 27061-1-APDownload protocol
IF protocol for PTGIS antibody 27061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols