PTGER3 Polyclonal antibody

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• PTGER3 Polyclonal antibody (24761-1-AP)

PTGER3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24761-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

EP3, EP3 I, EP3 II, EP3 III, EP3 IV

• 

  WB analysis of mouse brain using 24761-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24761-1-AP (PTGER3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse cerebellum using 24761-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 24761-1-AP (PTGER3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 24761-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24761-1-AP (PTGER3 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 24761-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24761-1-AP (PTGER3 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24761-1-AP targets PTGER3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21542

  Product name: Recombinant human PTGER3 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-100 aa of BC024229

  Sequence: MKETRGYGGDAPFCTRLNHSYTGMWAPERSAEARGNLTRPPGSGEDCGSVSVAFPITMLLTGFVGNALAMLLVSRSYRRRESKRKKSFLLCIGWLALTDL

• Full Name: prostaglandin E receptor 3 (subtype EP3)
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 40-48 kDa
• GenBank Accession Number: BC024229
• Gene Symbol: PTGER3
• Gene ID (NCBI): 5733
• RRID: AB_2879710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43115
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTGER3 (Prostaglandin E Receptor 3), also known as EP3, is a G protein-coupled receptor (GPCR). Activates pathways involved in proliferation, migration, and apoptosis, often via cross-talk with PI3K-AKT and MAPK cascades (PMID: 38566528). High PTGER3 levels drive tumorigenesis, angiogenesis, and cisplatin resistance via the Ras-MAPK/Erk-ETS1-ELK1/CFTR1 axis (PMID: 30655206).

Publications

What published studies show

The PTGER3 Polyclonal antibody (Cat# 24761-1-AP) has 2 citations. They appear in Cell Metabolism (2024) and Developmental Cell (2023). The research fields span metabolic regulation of insulin sensitivity via m6A mRNA methylation in brown fat, and chromatin remodeling of prostaglandin signaling in smooth muscle related to reproductive tract biology. Both studies used the antibody for Western blot in human and mouse samples.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB	Cell Metab; m6A mRNA methylation in brown fat regulates systemic insulin sensitivity via an inter-organ prostaglandin signaling axis independent of UCP1; Authors - Ling Xiao
mouse,human	WB	Dev Cell; Chromatin remodeling of prostaglandin signaling in smooth muscle enables mouse embryo passage through the female reproductive tract; Authors - Qiliang Xin