PTEN Monoclonal antibody

• 

  WB analysis using 60300-3-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60300-3-Ig (PTEN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

PTEN Monoclonal Antibody for WB, ELISA

Cat No. 60300-3-Ig

Clone No.4D8G9

Publications(2)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

10q23del, 4D8G9, Bannayan Zonana syndrome, BC005821, BZS

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, DU 145 cells, HT-29 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

60300-3-Ig targets PTEN in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PTEN fusion protein Ag17274
• Full Name: phosphatase and tensin homolog
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC005821
• Gene Symbol: PTEN
• Gene ID (NCBI): 5728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P60484
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTEN (also designated MMAC1), products of tumor suppressor genes, are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors, such as brain, breast, kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa.

Publications

Species	Application	Title
human	WB	J Immunother; Taurine Inhibits Lung Metastasis in Triple-Negative Breast Cancer by Modulating Macrophage Polarization Through PTEN-PI3K/Akt/mTOR Pathway; Authors - Yufeng Lin
human	IHC	Cell Death Discov; Myeloid PTEN loss affects the therapeutic response by promoting stress granule assembly and impairing phagocytosis by macrophages in breast cancer; Authors - Yan Li