PTDSS1 Polyclonal antibody

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  WB analysis using 20820-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20820-1-AP (PTDSS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20820-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PTDSS1 antibody (20820-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PTDSS1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 20820-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

PSS1, PSSA, PSS-1, Phosphatidylserine synthase 1, KIAA0024

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Tested Applications

• Positive WB detected in: HEK-293 cells, unboiled HEK-293 cells, HeLa cells, unboiled HeLa cells
• Positive IF/ICC detected in: HepG2 cells

It is best to use unboiled samples for detection, for membrane proteins are hydrophobic and tend to aggregate when boiled at high temperatures.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20820-1-AP targets PTDSS1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTDSS1 fusion protein Ag14170
• Full Name: phosphatidylserine synthase 1
• Calculated Molecular Weight: 473 aa, 56 kDa
• Observed Molecular Weight: 38~40 kDa
• GenBank Accession Number: BC002376
• Gene Symbol: PTDSS1
• Gene ID (NCBI): 9791
• RRID: AB_3669350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48651
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTDSS1 gene encodes the enzyme phosphatidylserine synthase 1 (PSS1), which catalyzes the production of phosphatidylserine (PS), a membrane phospholipid involved in different cellular processes such as development, cell signaling, apoptosis, and blood coagulation (PMID: 35224839). PTDSS1 is highly enriched in mitochondria-associated membranes (MAM) and is largely excluded from the bulk of the endoplasmic reticulum (ER). Low apparent molecular mass of 40 kDa compared with the size predicted was attributed to the exceptionally high content of hydrophobic amino acids in PSS1 (PMID: 10938271).