PTCH2 Polyclonal antibody

PTCH2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 55091-1-AP

Synonyms

UNQ560/PRO1121/PRO57079, PTC2, Protein patched homolog 2, patched homolog 2 (Drosophila), Patched 2

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Validation Data Gallery

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  WB analysis of HepG2 using 55091-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55091-1-AP (PTCH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 55091-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55091-1-AP (PTCH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

55091-1-AP targets PTCH2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: patched homolog 2 (Drosophila)
• Calculated Molecular Weight: 131 kDa
• Observed Molecular Weight: 131-140 kDa
• GenBank Accession Number: NM_003738
• Gene Symbol: PTCH2
• Gene ID (NCBI): 8643
• RRID: AB_10858392
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTCH1 (patched homolog 1) and PTCH2 are twelve-pass transmembrane proteins that act receptors of Hedgehog signaling pathway (PMID: 8906794; 9811851). Signals of Hedgehog family proteins (SHH, IHH and DHH in humans) are transduced through Patched family receptors (PTCH1 and PTCH2) and Smoothened (SMO) to GLI family transcription factors. Hedgehog pathway plays a critical role in embryonic development and tissue homeostasis, and its dysregulation has been implicated in many human diseases including congenital disorder and cancer (PMID: 23719536; 23532857). PTCH2 may function as a tumor suppressor in the Hedgehog signaling pathway. Alterations in PTCH2 gene have been associated with nevoid basal cell carcinoma syndrome, basal cell carcinoma, medulloblastoma, and susceptibility to congenital macrostomia. This antibody is specific to PTCH2.

Publications

Species	Application	Title
rat	WB	Exp Mol Med; Lentivirus-mediated silencing of the PTC1 and PTC2 genes promotes recovery from spinal cord injury by activating the Hedgehog signaling pathway in a rat model.; Authors - Ya-Dong Zhang
human	WB	iScience; 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone provokes progression from chronic pancreatitis to pancreatic intraepithelial neoplasia.; Authors - Xin Chen
mouse	WB	Cell Res; SCUBE2 mediates bone metastasis of luminal breast cancer by modulating immune-suppressive osteoblastic niches; Authors - Qiuyao Wu