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  • KD/KO Validated

PTCH1 Polyclonal antibody

PTCH1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 17520-1-AP


HPE7, NBCCS, Patched, patched homolog 1 (Drosophila), Patched1, Protein patched homolog 1, PTC, PTC1, PTCH, PTCH1, PTCH11

Tested Applications

Positive WB detected inmouse brain tissue, A431 cells, HeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17520-1-AP targets PTCH1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name patched homolog 1 (Drosophila)
Calculated Molecular Weight 161 kDa
Observed Molecular Weight 161 kDa
GenBank Accession NumberNM_000264
Gene Symbol PTCH1
Gene ID (NCBI) 5727
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTCH1 (patched homolog 1) is a twelve-pass transmembrane protein that acts as a receptor of Hedgehog signaling pathway (PMID: 8906794). Hedgehog pathway plays a critical role in embryonic development and tissue homeostasis, and its dysregulation has been implicated in many human diseases including congenital disorder and cancer (PMID: 23719536; 23532857). In the absence of Hedgehog proteins (SHH, IHH and DHH in humans), PTCH1 represses the activity of Smoothened (SMO). Binding of Hedgehog proteins to PTCH1 inhibits the repression of SMO, resulting in the activation of downstream targets through the Gli transcriptional effectors (PMID: 23719536; 17139287). The gene of PTCH1 is considered to be a tumor suppressor gene. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly.


Product Specific Protocols
WB protocol for PTCH1 antibody 17520-1-APDownload protocol
IHC protocol for PTCH1 antibody 17520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.

Authors - Rongxuan Zhu

Nat Commun

mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation.

Authors - Bo Yan

Am J Hum Genet

Genomic analyses reveal mutational signatures and frequently altered genes in esophageal squamous cell carcinoma.

Authors - Ling Zhang


Activation of Hedgehog signaling in mesenchymal stem cells induces cartilage and bone tumor formation via Wnt/β-Catenin.

Authors - Qi Deng

J Mol Cell Biol

Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression.

Authors - Yuwei Li

Cancers (Basel)

RNA-seq and ChIP-seq Identification of Unique and Overlapping Targets of GLI Transcription Factors in Melanoma Cell Lines

Authors - Matea Kurtović


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Charlotte (Verified Customer) (07-27-2022)

1/2 : WT starved and then ShhN stimulated. 3/4 : Ptch1 KO cells starved and then ShhN stimulated. It should be between the uppermost band and the following one from the ladder but we can conclude that there is nothing and the antibody is really dirty

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: NIH-3T3
PTCH1 Antibody Western Blot validation (1/500 dilution) in NIH-3T3 (Cat no:17520-1-AP)

Boyan (Verified Customer) (04-04-2019)

The band at around 200 KD is not the true band of Ptc1, because this band cannot be increased by treating the cells with Shh ligand, and did not disappear in Ptc1 KO cells. As positive controls, Gli1 was induced by treating the cells with the Shh ligand.

  • Applications: Western Blot,

Juan Pablo (Verified Customer) (11-29-2018)

I see a band around 200kDa kind of clean. Never detected the 149kDa shown in proteintech website.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cerebellum from postnatal pups P2 to P14
PTCH1 Antibody Western Blot, validation (1:1000 dilution) in Cerebellum from postnatal pups P2 to P14 (Cat no:17520-1-AP)