PTCH1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PTCH1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

28

Reviews (3)

Cat no : 17520-1-AP

Synonyms

HPE7, NBCCS, Patched, patched homolog 1 (Drosophila), Patched1, Protein patched homolog 1, PTC, PTC1, PTCH, PTCH1, PTCH11

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Validation Data Gallery

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  WB analysis of HeLa using 17520-1-AP

  WB result of PTCH1 antibody (17520-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTCH1 transfected HeLa cells.

• 

  WB analysis of mouse brain using 17520-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17520-1-AP (PTCH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17520-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17520-1-AP (PTCH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17520-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17520-1-AP (PTCH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 17520-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17520-1-AP (PTCH1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 17520-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17520-1-AP (PTCH1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, A431 cells, HeLa cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 21 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Product Information

17520-1-AP targets PTCH1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: patched homolog 1 (Drosophila)
• Calculated Molecular Weight: 161 kDa
• Observed Molecular Weight: 161 kDa
• GenBank Accession Number: NM_000264
• Gene Symbol: PTCH1
• Gene ID (NCBI): 5727
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTCH1 (patched homolog 1) is a twelve-pass transmembrane protein that acts as a receptor of Hedgehog signaling pathway (PMID: 8906794). Hedgehog pathway plays a critical role in embryonic development and tissue homeostasis, and its dysregulation has been implicated in many human diseases including congenital disorder and cancer (PMID: 23719536; 23532857). In the absence of Hedgehog proteins (SHH, IHH and DHH in humans), PTCH1 represses the activity of Smoothened (SMO). Binding of Hedgehog proteins to PTCH1 inhibits the repression of SMO, resulting in the activation of downstream targets through the Gli transcriptional effectors (PMID: 23719536; 17139287). The gene of PTCH1 is considered to be a tumor suppressor gene. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly.

Publications

Species	Application	Title
human	WB	Nat Commun; TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.; Authors - Rongxuan Zhu
mouse	WB	Nat Commun; mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation.; Authors - Bo Yan
mouse	IHC	Am J Hum Genet; Genomic analyses reveal mutational signatures and frequently altered genes in esophageal squamous cell carcinoma.; Authors - Ling Zhang
human	WB	Elife; Activation of Hedgehog signaling in mesenchymal stem cells induces cartilage and bone tumor formation via Wnt/β-Catenin.; Authors - Qi Deng
mouse	WB	J Mol Cell Biol; Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression.; Authors - Yuwei Li
human	WB	Cancers (Basel); RNA-seq and ChIP-seq Identification of Unique and Overlapping Targets of GLI Transcription Factors in Melanoma Cell Lines; Authors - Matea Kurtović

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (07-27-2022)

1/2 : WT starved and then ShhN stimulated. 3/4 : Ptch1 KO cells starved and then ShhN stimulated. It should be between the uppermost band and the following one from the ladder but we can conclude that there is nothing and the antibody is really dirty

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: NIH-3T3

PTCH1 Antibody Western Blot validation (1/500 dilution) in NIH-3T3 (Cat no:17520-1-AP)

FH

Boyan (Verified Customer) (04-04-2019)

The band at around 200 KD is not the true band of Ptc1, because this band cannot be increased by treating the cells with Shh ligand, and did not disappear in Ptc1 KO cells. As positive controls, Gli1 was induced by treating the cells with the Shh ligand.

• Applications: Western Blot,

FH

Juan Pablo (Verified Customer) (11-29-2018)

I see a band around 200kDa kind of clean. Never detected the 149kDa shown in proteintech website.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cerebellum from postnatal pups P2 to P14

PTCH1 Antibody Western Blot, validation (1:1000 dilution) in Cerebellum from postnatal pups P2 to P14 (Cat no:17520-1-AP)

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