|Positive WB detected in||mouse brain tissue, A431 cells, HeLa cells|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Sample-dependent, check data in validation data gallery|
17520-1-AP targets PTCH1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||patched homolog 1 (Drosophila)|
|Calculated molecular weight||161 kDa|
|Observed molecular weight||161 kDa|
|GenBank accession number||NM_000264|
|Gene ID (NCBI)||5727|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PTCH1 (patched homolog 1) is a twelve-pass transmembrane protein that acts as a receptor of Hedgehog signaling pathway (PMID: 8906794). Hedgehog pathway plays a critical role in embryonic development and tissue homeostasis, and its dysregulation has been implicated in many human diseases including congenital disorder and cancer (PMID: 23719536; 23532857). In the absence of Hedgehog proteins (SHH, IHH and DHH in humans), PTCH1 represses the activity of Smoothened (SMO). Binding of Hedgehog proteins to PTCH1 inhibits the repression of SMO, resulting in the activation of downstream targets through the Gli transcriptional effectors (PMID: 23719536; 17139287). The gene of PTCH1 is considered to be a tumor suppressor gene. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Charlotte (Verified Customer) (07-27-2022)
1/2 : WT starved and then ShhN stimulated. 3/4 : Ptch1 KO cells starved and then ShhN stimulated. It should be between the uppermost band and the following one from the ladder but we can conclude that there is nothing and the antibody is really dirty
Boyan (Verified Customer) (04-04-2019)
The band at around 200 KD is not the true band of Ptc1, because this band cannot be increased by treating the cells with Shh ligand, and did not disappear in Ptc1 KO cells. As positive controls, Gli1 was induced by treating the cells with the Shh ligand.
Juan Pablo (Verified Customer) (11-29-2018)
I see a band around 200kDa kind of clean. Never detected the 149kDa shown in proteintech website.