PSMD9 Polyclonal antibody

• Featured Product
• KD/KO Validated

PSMD9 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 26922-1-AP

Synonyms

Rpn4, p27, 26S proteasome non-ATPase regulatory subunit 9

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Validation Data Gallery

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  WB analysis of A549 using 26922-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 26922-1-AP

  WB result of PSMD9 antibody (26922-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD9 transfected A549 cells.

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  WB analysis of mouse spleen using 26922-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 26922-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 26922-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 26922-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 26922-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 26922-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26922-1-AP (PSMD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 26922-1-AP

  IP result of anti-PSMD9 (IP:26922-1-AP, 4ug; Detection:26922-1-AP 1:400) with A549 cells lysate 1330 ug.

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  IHC staining of human breast cancer using 26922-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26922-1-AP (PSMD9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26922-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26922-1-AP (PSMD9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 26922-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PSMD9 antibody (26922-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 26922-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug Anti-Human PSMD9 (26922-1-AP)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells, MDA-MB-453s cells, HepG2 cells, Jurkat cells, mouse spleen tissue
• Positive IP detected in: A549 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26922-1-AP targets PSMD9 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMD9 fusion protein Ag25638
• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 9
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC004213
• Gene Symbol: PSMD9
• Gene ID (NCBI): 5715
• RRID: AB_2880687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMD9 is a ubiquitous protein of eukaryotic cells and is a chaperon of the 26S proteasome complex, which degrades ubiquitinated proteins in eukaryotic cells and contributes to the degradation of intracellular proteins into antigenic peptides for antigen presentation by MHC class I cells. The 26S mammalian base sub-complex involves three distinct modules which have ATPase subunits distinctly associated to three chaperones, one of which is PSMD9 regulating the modules assembly. The PSMD9 ubiquitous regulatory role within the proteasome implies its potential pleiotropic effects within different physio-pathological systems. PSMD9 is known to form a stable subcomplex with PSMC3 and PSMC6, two of the AAA-ATPases, assisting in the assembly of the 20S and 19S particles to form the holo complex.