PSMD8 Polyclonal antibody

PSMD8 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27504-1-AP

Synonyms

HIP6, HYPF, Nin1p, p31, PSMD8, Rpn12, S14

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Validation Data Gallery

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  WB analysis using 27504-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27504-1-AP (PSMD8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 27504-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PSMD8 antibody (27504-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27504-1-AP targets PSMD8 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMD8 fusion protein Ag26567
• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 8
• Calculated Molecular Weight: 257 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC001164
• Gene Symbol: PSMD8
• Gene ID (NCBI): 5714
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Proteasome 26S Subunit, Non-ATPase (PSMD) gene family, which includes the PSMD1 to PSMD14, regulate ubiqutinated protein breakdown in circulation as well as tumorigenesis. Studies have shown that PSMD8 interacts with the sperm adhesin AQN1 to limit polyfertilization. PSMD8 was more expressed in normal skeletal muscle, cardiac muscle, and tongue muscle; among malignant tumors, PSMD8 was expressed in ovarian cancer, testicular cancer, glioma, and melanoma(PMID:37349676).