PSMD4 Monoclonal antibody, PBS Only

PSMD4 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G1A1

Cat no : 66179-1-PBS

Synonyms

AF, AF 1, Antisecretory factor 1, ASF, MCB1, PSMD4, pUB R5, Rpn10, S5A

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Validation Data Gallery

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  WB analysis using 66179-1-Ig (same clone as 66179-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66179-1-Ig (PSMD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66179-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66179-1-Ig (same clone as 66179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66179-1-Ig (PSMD4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66179-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66179-1-Ig (same clone as 66179-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66179-1-Ig (PSMD4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66179-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66179-1-Ig (same clone as 66179-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMD4 antibody (66179-1-Ig, Clone: 3G1A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66179-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66179-1-PBS targets PSMD4 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSMD4 fusion protein Ag7010
• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC002365
• Gene Symbol: PSMD4
• Gene ID (NCBI): 5710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.