PSMD13 Polyclonal antibody

• Featured Product
• KD/KO Validated

PSMD13 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15261-1-AP

Synonyms

S11, Rpn9, p40.5, HSPC027, 26S proteasome non-ATPase regulatory subunit 13

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Validation Data Gallery

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  WB analysis of HEK-293 using 15261-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15261-1-AP (PSMD13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293T using 15261-1-AP

  IP result of anti-PSMD13 (IP:15261-1-AP, 4ug; Detection:15261-1-AP 1:1000) with HEK-293T cells lysate 1200 ug.

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  IHC staining of human kidney using 15261-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15261-1-AP (PSMD13 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 15261-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15261-1-AP (PSMD13 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 15261-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15261-1-AP (PSMD13 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15261-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15261-1-AP (PSMD13 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15261-1-AP

  Immunofluorescent analysis of Hela cells, using PSMD13 antibody 15261-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells, Y79 cells
• Positive IP detected in: HEK-293T cells
• Positive IHC detected in: human kidney tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

15261-1-AP targets PSMD13 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMD13 fusion protein Ag7257
• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 13
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC001100
• Gene Symbol: PSMD13
• Gene ID (NCBI): 5719
• RRID: AB_2172977
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	J Neurosci; Pharmacological Amelioration of Cone Survival and Vision in a Mouse Model for Leber Congenital Amaurosis.; Authors - Songhua Li ; KD Validated
mouse	WB,IHC	J Biol Chem; Rescue of enzymatic function for disease-associated RPE65s containing various missense mutations in non-active sites.; Authors - Songhua Li
	WB	J Biochem; Temperature sensitive retinoid isomerase activity of RPE65 mutants associated with Leber congenital amaurosis.; Authors - Songhua Li
mouse	WB	bioRxiv; Proteasome gene expression is controlled by the coordinated functions of multiple transcription factors; Authors - Jennifer E Gilda