PSMD11 Polyclonal antibody

• Featured Product
• KD/KO Validated

PSMD11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 14786-1-AP

Synonyms

S9, Rpn6, p44.5, 26S proteasome non-ATPase regulatory subunit 11

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Validation Data Gallery

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  WB analysis of HeLa using 14786-1-AP

  WB result of PSMD11 antibody (14786-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD11 transfected HeLa cells.

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  WB analysis of HeLa using 14786-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14786-1-AP (PSMD11 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14786-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14786-1-AP (PSMD11 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 14786-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14786-1-AP (PSMD11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 14786-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14786-1-AP (PSMD11 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 14786-1-AP

  IP result of anti-PSMD11 (IP:14786-1-AP, 3ug; Detection:14786-1-AP 1:500) with MCF-7 cells lysate 2000ug.

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  IHC staining of human stomach cancer using 14786-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14786-1-AP (PSMD11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14786-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14786-1-AP (PSMD11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat lung using 14786-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 14786-1-AP (PSMD11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 14786-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 14786-1-AP (PSMD11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 14786-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14786-1-AP (PSMD11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 14786-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14786-1-AP (PSMD11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 14786-1-AP

  Immunofluorescent analysis of MCF-7 cells using 14786-1-AP (PSMD11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, mouse testis tissue, rat brain tissue
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human stomach cancer tissue, human lung tissue, rat lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

14786-1-AP targets PSMD11 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMD11 fusion protein Ag6435
• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 11
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC000437
• Gene Symbol: PSMD11
• Gene ID (NCBI): 5717
• RRID: AB_2268979
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 26 S proteasome is a 2.5-MDa molecular machine that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core particle and two 19 S regulatory particles (RPs) composed of 6 ATPase (RPT) and 13 non-ATPase (RPN) subunits. PSMD11 gene encodes 19S proteasome subunit RPN6. Increased levels of PSMD11 and a corresponding increased assembly of the 26S/30S proteasome is correlated with high proteasome activity. In vitro ectopic expression of PSMD11 is sufficient to increase proteasome assembly and activity. Latest research has implicated PSMD11 in regulation of human embryonic stem cells function.

Publications

Species	Application	Title
human	WB	Oncogene; The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.; Authors - Sparks A A
human	WB	Mol Cell Proteomics; Proteomic Profiling Identified Multiple Short-lived Members of the Central Proteome as the Direct Targets of the Addicted oncogenes in Cancer Cells.; Authors - Qi Tong-Gang TG
rat	WB	J Med Chem; Target Fishing Reveals a Novel Mechanism of 1,2,4-Oxadiazole Derivatives Targeting Rpn6, a Subunit of 26S Proteasome.; Authors - Zhen Dai
mouse	WB	FASEB J; Susceptibility of microtubule-associated protein 1 light chain 3β (MAP1LC3B/ LC3B) knockout mice to lung injury and fibrosis.; Authors - Vidya Sagar Kesireddy
human	WB	J Cell Biochem; Homoharringtonine could induce quick protein synthesis of PSMD11 through activating MEK1/ERK1/2 signaling pathway in pancreatic cancer cells.; Authors - Lele Wang
mouse	WB,IF	BMC Dev Biol; Generation and identification of a conditional knockout allele for the PSMD11 gene in mice.; Authors - Linlin Zhao