PSMB9 Polyclonal antibody

• Featured Product
• KD/KO Validated

PSMB9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 14544-1-AP

Synonyms

LMP2, EC:3.4.25.1, RING12, PSMB6i, Proteasome subunit beta-1i

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Validation Data Gallery

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  WB analysis using 14544-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14544-1-AP (PSMB9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 14544-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14544-1-AP (LMP2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 14544-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 14544-1-AP (LMP2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14544-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14544-1-AP (PSMB9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 14544-1-AP

  IP result of anti-PSMB9 (IP:14544-1-AP, 4ug; Detection:14544-1-AP 1:300) with Raji cells lysate 2800ug.

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  IHC staining of human spleen using 14544-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14544-1-AP (PSMB9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse bladder using 14544-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 14544-1-AP (PSMB9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 14544-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 14544-1-AP (PSMB9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 14544-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 14544-1-AP (PSMB9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 14544-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 14544-1-AP (PSMB9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14544-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 14544-1-AP (LMP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 14544-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 14544-1-AP (LMP2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 14544-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 14544-1-AP (LMP2 antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human tonsillitis using 14544-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 14544-1-AP (LMP2 antibody) at dilution of 1:25 (under 40x lens).

• 

  IF Staining of HepG2 using 14544-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 14544-1-AP (LMP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HL-60 cells, rat spleen tissue, mouse kidney tissue, mouse liver tissue, mouse lung tissue, mouse spleen tissue, rat liver tissue, Raji cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human spleen tissue, human tonsillitis tissue, human renal cell carcinoma tissue, mouse bladder tissue, mouse stomach tissue, rat stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

14544-1-AP targets PSMB9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMB9 fusion protein Ag6048
• Full Name: proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2)
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 20-23 kDa
• GenBank Accession Number: BC065513
• Gene Symbol: PSMB9
• Gene ID (NCBI): 5698
• RRID: AB_2268925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMB9, also named as LMP2, PSMB6i and RING12, belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. High expression of PSMB9 associated with the poor prognosis of patients with NPC.

Publications

Species	Application	Title
human	WB	Mol Cell; DNA damage promotes HLA class I presentation by stimulating a pioneer round of translation-associated antigen production.; Authors - Yuki Uchihara ; KD Validated
human	IF	Cell Res; In-cell infection: a novel pathway for Epstein-Barr virus infection mediated by cell-in-cell structures.; Authors - Chao Ni
mouse		EMBO J; Proteasomal degradation within endocytic organelles mediates antigen cross-presentation.; Authors - Debrup Sengupta
human	WB	Cancer Lett; Deciphering the tumor-suppressive role of PSMB9 in melanoma through multi-omics and single-cell transcriptome analyses; Authors - Xing Hu
mouse	WB	Cell Rep; The portrait of liver cancer is shaped by mitochondrial genetics.; Authors - Mrittika Chattopadhyay
human	IF,WB	Front Oncol; Immune Characteristics and Prognosis Analysis of the Proteasome 20S Subunit Beta 9 in Lower-Grade Gliomas.; Authors - Junzhe Liu