PSMB8 Monoclonal antibody

PSMB8 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2A5B6

Cat no : 66759-1-Ig

Synonyms

Proteasome subunit beta-5i, Proteasome subunit beta type-8, Proteasome subunit beta type 8, LMP7, EC:3.4.25.1



Tested Applications

Positive WB detected inJurkat cells, HSC-T6 cells, Raji cells, Ramos cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue, human oesophagus cancer tissue, mouse colon tissue, mouse stomach tissue, rat colon tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lung cancer tissue, human liver cancer tissue
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66759-1-Ig targets PSMB8 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSMB8 fusion protein Ag6780
Full Name proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC001114
Gene Symbol PSMB8
Gene ID (NCBI) 5696
RRIDAB_2882105
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMB8(Proteasome subunit beta type-8) is also named as LMP7, PSMB5i, RING10, Y2 and belongs to the peptidase T1B family. The gene encodes the chymotrypsin-like catalytic subunit of the immunoproteasome(PMID: 19525961). PSMB8 has a role in controlling pathogenic immune responses and may be a target in autoimmune disorders. Its prosequence is not essential for incorporation of PSMB8 into the maturing proteasome, but it increased the efficiency of PSMB8 incorporation and proteasome maturation(PMID: 10926487). The pro-PSMB8 is a 276aa protein with the molecular mass of 30 kDa, and the mature form is about 23kDa due to the 72aa propeptide cleaved. Defects in PSMB8 are the cause of Nakajo syndrome (NKJO).

Protocols

Product Specific Protocols
WB protocol for PSMB8 antibody 66759-1-IgDownload protocol
IHC protocol for PSMB8 antibody 66759-1-IgDownload protocol
IF protocol for PSMB8 antibody 66759-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols