PSMB8 Monoclonal antibody
PSMB8 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
2A5B6
Cat no : 66759-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, HSC-T6 cells, Raji cells, Ramos cells |
Positive IHC detected in | human lung cancer tissue, human liver cancer tissue, human oesophagus cancer tissue, mouse colon tissue, mouse stomach tissue, rat colon tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human lung cancer tissue, human liver cancer tissue |
Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66759-1-Ig targets PSMB8 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | PSMB8 fusion protein Ag6780 |
Full Name | proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7) |
Calculated Molecular Weight | 30 kDa |
Observed Molecular Weight | 23 kDa |
GenBank Accession Number | BC001114 |
Gene Symbol | PSMB8 |
Gene ID (NCBI) | 5696 |
RRID | AB_2882105 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PSMB8(Proteasome subunit beta type-8) is also named as LMP7, PSMB5i, RING10, Y2 and belongs to the peptidase T1B family. The gene encodes the chymotrypsin-like catalytic subunit of the immunoproteasome(PMID: 19525961). PSMB8 has a role in controlling pathogenic immune responses and may be a target in autoimmune disorders. Its prosequence is not essential for incorporation of PSMB8 into the maturing proteasome, but it increased the efficiency of PSMB8 incorporation and proteasome maturation(PMID: 10926487). The pro-PSMB8 is a 276aa protein with the molecular mass of 30 kDa, and the mature form is about 23kDa due to the 72aa propeptide cleaved. Defects in PSMB8 are the cause of Nakajo syndrome (NKJO).
Protocols
Product Specific Protocols | |
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WB protocol for PSMB8 antibody 66759-1-Ig | Download protocol |
IHC protocol for PSMB8 antibody 66759-1-Ig | Download protocol |
IF protocol for PSMB8 antibody 66759-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |