PSMB8 Polyclonal antibody

• Featured Product
• KD/KO Validated

PSMB8 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 14859-1-AP

Synonyms

beta5i, D6S216, D6S216E, LMP7, Low molecular mass protein 7, Macropain subunit C13, Proteasome component C13, Proteasome subunit beta 5i, Proteasome subunit beta type 8, PSMB5i, PSMB8, RING10, Y2

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Validation Data Gallery

• 

  WB analysis of mouse kidney using 14859-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14859-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14859-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14859-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14859-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14859-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14859-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14859-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14859-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14859-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14859-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14859-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14859-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14859-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14859-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 14859-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14859-1-AP (LMP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 14859-1-AP

  IP result of anti-PSMB8 (IP:14859-1-AP, 3ug; Detection:14859-1-AP 1:1000) with Raji cells lysate 2800ug.

• 

  IHC staining of human lymphoma using 14859-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14859-1-AP (LMP7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 14859-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14859-1-AP (LMP7 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, human kidney tissue, Jurkat cells, Raji cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

14859-1-AP targets PSMB8 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMB8 fusion protein Ag6660
• Full Name: proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7)
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC001114
• Gene Symbol: PSMB8
• Gene ID (NCBI): 5696
• RRID: AB_2268923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMB8(Proteasome subunit beta type-8) is also named as LMP7, PSMB5i, RING10, Y2 and belongs to the peptidase T1B family. The gene encodes the chymotrypsin-like catalytic subunit of the immunoproteasome(PMID: 19525961). PSMB8 has a role in controlling pathogenic immune responses and may be a target in autoimmune disorders. Its prosequence is not essential for incorporation of PSMB8 into the maturing proteasome, but it increased the efficiency of PSMB8 incorporation and proteasome maturation(PMID: 10926487). The pro-PSMB8 is a 276aa protein with the molecular mass of 30 kDa, and the mature form is about 23kDa due to the 72aa propeptide cleaved. Defects in PSMB8 are the cause of Nakajo syndrome (NKJO).

Publications

Species	Application	Title
human	WB	J Cell Mol Med; miR-451a inhibits cancer growth, epithelial-mesenchymal transition and induces apoptosis in papillary thyroid cancer by targeting PSMB8.; Authors - Xinlong Fan
mouse	WB,IHC	J Biol Chem; PITHD1 is a proteasome-interacting protein essential for male fertilization.; Authors - Hiroyuki Kondo
mouse	WB	JCI Insight; Blockade of the CXCR3/CXCL10 axis ameliorates inflammation caused by immunoproteasome dysfunction.; Authors - Yuki Sasaki
human,mouse	WB	Sci Rep; Airway epithelial immunoproteasome subunit LMP7 protects against rhinovirus infection; Authors - Kris Genelyn Dimasuay ; KO Validated
		Int Immunopharmacol; Targeted inhibition of the immunoproteasome blocks endothelial MHC class II antigen presentation to CD4+ T cells in chronic liver injury; Authors - Yuwei Zhang
mouse	WB	J Neuroinflammation; β-arrestin1 regulates astrocytic reactivity via Drp1-dependent mitochondrial fission: implications in postoperative delirium; Authors - Fuzhou Hua