PSMB7 Polyclonal antibody, PBS Only

PSMB7 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30283-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 30283-1-AP (same clone as 30283-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30283-1-AP (PSMB7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30283-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 30283-1-AP (same clone as 30283-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30283-1-AP (PSMB7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30283-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 30283-1-AP (same clone as 30283-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30283-1-AP (PSMB7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30283-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 30283-1-AP (same clone as 30283-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30283-1-AP (PSMB7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30283-1-PBS in a different storage buffer formulation.

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Product Information

30283-1-PBS targets PSMB7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMB7 fusion protein Ag32201
• Full Name: proteasome (prosome, macropain) subunit, beta type, 7
• Calculated Molecular Weight: 29.9 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC000509
• Gene Symbol: PSMB7
• Gene ID (NCBI): 5695
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. PSMB7 protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. Component of the 20S core proteasome complex are involved in the proteolytic degradation of most intracellular proteins. PSMB7 displays a trypsin-like activity.(PMID:15244466, PMID:27176742, PMID: 8610016)