PSMB2 Monoclonal antibody

• Featured Product
• KD/KO Validated

PSMB2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3C5C5

Cat no : 68180-1-Ig

Synonyms

HC7 I, Macropain subunit C7 I, Proteasome component C7 I, Proteasome subunit beta type 2, PSMB2

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Validation Data Gallery

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  WB analysis using 68180-1-Ig

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 68180-1-Ig (PSMB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68180-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68180-1-Ig (PSMB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of HeLa using 68180-1-Ig

  WB result of PSMB2 antibody (68180-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMB2 transfected HeLa cells.

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  IF Staining of HCT 116 using 68180-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using PSMB2 antibody (68180-1-Ig, Clone: 3C5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 68180-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMB2 antibody (68180-1-Ig, Clone: 3C5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of PC-3 using 68180-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using PSMB2 antibody (68180-1-Ig, Clone: 3C5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, SKOV-3 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IF/ICC detected in: HepG2 cells, HCT 116 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68180-1-Ig targets PSMB2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSMB2 fusion protein Ag7308
• Full Name: proteasome (prosome, macropain) subunit, beta type, 2
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC000268
• Gene Symbol: PSMB2
• Gene ID (NCBI): 5690
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMB2(proteasome subunit beta type-2) is also named as macropain subunit C7-I, proteasome component C7-I, multicatalytic endopeptidase complex subunit C7-I and belongs to the peptidase T1B family. It is involved in an ATP/ubiquitin-dependent non-lysosomal proteolytic pathway. The up-regulation of PSMB2 may indicate the activated neuronal defensive mechanism in VAD(vitamin A depletion) brain regions, which may underlie the VAD-related psychosis behavior(PMID:21190828).