PSMB10 Monoclonal antibody, PBS Only

PSMB10 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G8C1

Cat no : 66403-1-PBS

Synonyms

beta2i, LMP10, Low molecular mass protein 10, Macropain subunit MECl 1, MECL1, Proteasome MECl 1, Proteasome subunit beta 2i, PSMB10

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Validation Data Gallery

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  WB analysis using 66403-1-Ig (same clone as 66403-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66403-1-Ig (same clone as 66403-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat thymus using 66403-1-Ig (same clone as 66403-1-PBS)

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 66403-1-Ig (same clone as 66403-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig thymus using 66403-1-Ig (same clone as 66403-1-PBS)

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Ramos using 66403-1-Ig (same clone as 66403-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 66403-1-Ig (same clone as 66403-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66403-1-Ig (same clone as 66403-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66403-1-Ig (PSMB10 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66403-1-Ig (same clone as 66403-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66403-1-Ig (PSMB10 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66403-1-PBS in a different storage buffer formulation.

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Product Information

66403-1-PBS targets PSMB10 in WB, IHC, Indirect ELISA applications and shows reactivity with human, pig, rat samples.

• Tested Reactivity: human, pig, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSMB10 fusion protein Ag8835
• Full Name: proteasome (prosome, macropain) subunit, beta type, 10
• Calculated Molecular Weight: 273 aa, 29 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC017198
• Gene Symbol: PSMB10
• Gene ID (NCBI): 5699
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SMB10(Proteasome subunit beta type-10) is also named as LMP10, MECL1.It belongs to the peptidase T1B family and is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.