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PSMA7 Polyclonal antibody
PSMA7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 15219-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, HeLa cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:3000-1:15000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 10 publications below |
Product Information
15219-1-AP targets PSMA7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PSMA7 fusion protein Ag7389 |
Full Name | proteasome (prosome, macropain) subunit, alpha type, 7 |
Calculated Molecular Weight | 28 kDa |
Observed Molecular Weight | 28-30 kDa |
GenBank Accession Number | BC004427 |
Gene Symbol | PSMA7 |
Gene ID (NCBI) | 5688 |
RRID | AB_2171751 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PSMA7(Proteasome subunit alpha type-7) is also named HSPC and belongs to the peptidase T1A family. It is an α-type subunit of the 20S proteasome core complex and participates in degrading proteins through the ubiquitin-proteasome pathway (UPP) which plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis(PMID: 19442227).
Protocols
Product Specific Protocols | |
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WB protocol for PSMA7 antibody 15219-1-AP | Download protocol |
IHC protocol for PSMA7 antibody 15219-1-AP | Download protocol |
IF protocol for PSMA7 antibody 15219-1-AP | Download protocol |
IP protocol for PSMA7 antibody 15219-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Brain Raising cGMP restores proteasome function and myelination in mice with a proteotoxic neuropathy. | ||
Glia Impairment of protein degradation and proteasome function in hereditary neuropathies. | ||
Mol Cell Proteomics Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7. | ||
Biochim Biophys Acta Mol Basis Dis Defective proteasome biogenesis into skin fibroblasts isolated from Rett syndrome subjects with MeCP2 non-sense mutations. | ||
Exp Cell Res The effect and mechanism of 19S proteasome PSMD11/Rpn6 subunit in D-Galactose induced mimetic aging models. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Janelle (Verified Customer) (09-12-2022) | Specific to target
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