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PSMA7 Polyclonal antibody

PSMA7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15219-1-AP

Synonyms

XAPC7, RC6 1, Proteasome subunit RC6-1, Proteasome subunit alpha type-7, HSPC



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:15000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 10 publications below

Product Information

15219-1-AP targets PSMA7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMA7 fusion protein Ag7389
Full Name proteasome (prosome, macropain) subunit, alpha type, 7
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC004427
Gene Symbol PSMA7
Gene ID (NCBI) 5688
RRIDAB_2171751
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMA7(Proteasome subunit alpha type-7) is also named HSPC and belongs to the peptidase T1A family. It is an α-type subunit of the 20S proteasome core complex and participates in degrading proteins through the ubiquitin-proteasome pathway (UPP) which plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis(PMID: 19442227).

Protocols

Product Specific Protocols
WB protocol for PSMA7 antibody 15219-1-APDownload protocol
IHC protocol for PSMA7 antibody 15219-1-APDownload protocol
IF protocol for PSMA7 antibody 15219-1-APDownload protocol
IP protocol for PSMA7 antibody 15219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Brain

Raising cGMP restores proteasome function and myelination in mice with a proteotoxic neuropathy.

Authors - Jordan J S VerPlank
mouseWB

Glia

Impairment of protein degradation and proteasome function in hereditary neuropathies.

Authors - Jordan J S VerPlank
humanWB

Mol Cell Proteomics

Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7.

Authors - Tatjana Goebel
humanWB

Biochim Biophys Acta Mol Basis Dis

Defective proteasome biogenesis into skin fibroblasts isolated from Rett syndrome subjects with MeCP2 non-sense mutations.

Authors - Diego Sbardella
humanWB

Cell Physiol Biochem

PSMA7 Directly Interacts with NOD1 and Regulates its Function.

Authors - Yang Liuzhong L
ratWB

Exp Cell Res

The effect and mechanism of 19S proteasome PSMD11/Rpn6 subunit in D-Galactose induced mimetic aging models.

Authors - Han Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Janelle (Verified Customer) (09-12-2022)

Specific to target

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000