PSMA4 Monoclonal antibody

PSMA4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Rabbit, Rat, Mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E6E4

Cat no : 68203-1-Ig

Synonyms

HC9, HsT17706, Macropain subunit C9, Proteasome component C9, Proteasome subunit L, PSC9, PSMA4

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Validation Data Gallery

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  WB analysis using 68203-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68203-1-Ig (PSMA4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 68203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68203-1-Ig (PSMA4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 68203-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PSMA4 antibody (68203-1-Ig, Clone: 2E6E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, K-562 cells, Pig heart, Rabbit heart, Rat heart, Mouse heart
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68203-1-Ig targets PSMA4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Pig, Rabbit, Rat, Mouse samples.

• Tested Reactivity: Human, Pig, Rabbit, Rat, Mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSMA4 fusion protein Ag32626
• Full Name: proteasome (prosome, macropain) subunit, alpha type, 4
• Calculated Molecular Weight: 237 aa, 27 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC056249
• Gene Symbol: PSMA4
• Gene ID (NCBI): 5685
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.