PSMA1 Monoclonal antibody, PBS Only

PSMA1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D1D5

Cat no : 67910-1-PBS

Synonyms

30 kDa prosomal protein, HC2, Macropain subunit C2, NU, PROS 30, PROS30, Proteasome component C2, Proteasome nu chain, PSC2, PSMA1

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Validation Data Gallery

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  WB analysis using 67910-1-Ig (same clone as 67910-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67910-1-Ig (PSMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67910-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 67910-1-Ig (same clone as 67910-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67910-1-Ig (PSMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67910-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67910-1-Ig (same clone as 67910-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67910-1-Ig (PSMA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67910-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67910-1-Ig (same clone as 67910-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67910-1-Ig (PSMA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67910-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67910-1-Ig (same clone as 67910-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67910-1-Ig (PSMA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67910-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67910-1-PBS targets PSMA1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSMA1 fusion protein Ag30527
• Full Name: proteasome (prosome, macropain) subunit, alpha type, 1
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 29-33 kDa
• GenBank Accession Number: BC015105
• Gene Symbol: PSMA1
• Gene ID (NCBI): 5682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PSMA1(Proteasome subunit alpha type-1) is also named as HC2, NU, PROS30, PSC2 and belongs to the peptidase T1A family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It is induced in breast cancer tissue and up-regulated in liver tumor tissues(PMID:17127214;16317774;17004105).