PSIP1 Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 67723-1-Ig (same clone as 67723-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67723-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67723-1-Ig (same clone as 67723-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67723-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67723-1-Ig (same clone as 67723-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67723-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 67723-1-Ig (same clone as 67723-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67723-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67723-1-Ig (same clone as 67723-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67723-1-Ig (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67723-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 67723-1-Ig (same clone as 67723-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSIP1 antibody (67723-1-Ig, Clone: 1E9E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67723-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50375-1

  Cytometric bead array standard curve of MP50375-1, PSIP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67723-1-PBS. Detection antibody: 68941-1-PBS. Standard:Ag21886. Range: 3.125-200 ng/mL.

PSIP1 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 67723-1-PBS

Clone No.1E9E7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

Lens epithelium-derived growth factor, LEDGF, DFS70, CLL-associated antigen KW-7, CLL associated antigen KW 7

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Product Information

67723-1-PBS targets PSIP1 as part of a matched antibody pair:

MP50375-1: 67723-1-PBS capture and 68941-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSIP1 fusion protein Ag30562
• Full Name: PC4 and SFRS1 interacting protein 1
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: NM_021144
• Gene Symbol: PSIP1
• Gene ID (NCBI): 11168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O75475
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

PSIP1, also known as PC4 and SRSF1 Interacting Protein 1 or Lens Epithelium-Derived Growth Factor (LEDGF), is a transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. It also plays a role in lens epithelial cell gene regulation and stress responses. It is a chromatin-associated protein with histone chaperone activity, involved in transcriptional elongation. PSIP1 may act as an adapter to coordinate pre-mRNA splicing. PSIP1 encodes two protein isoforms with molecular masses of 52 and 75 kDa. p52 and p75 were identified as interacting with transcription factor PC4 and shown to act as transcriptional coactivators.