PSENEN Recombinant antibody

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  WB analysis using 83818-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83818-1-RR (PSENEN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83818-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83818-1-RR against Human PSENEN were performed. The affinity constant is below 1 pM.

PSENEN {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 83818-1-RR

Clone No.240596H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Presenilin enhancer protein 2, PEN2, PEN 2, Gamma-secretase subunit PEN-2, Gamma secretase subunit PEN 2

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Tested Applications

• Positive WB detected in: A549 cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83818-1-RR targets PSENEN in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PSENEN fusion protein Ag9243
• Full Name: presenilin enhancer 2 homolog (C. elegans)
• Calculated Molecular Weight: 101 aa, 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC009575
• Gene Symbol: PSENEN
• Gene ID (NCBI): 55851
• RRID: AB_3671404
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9NZ42
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSENEN, also named PEN2, is a component of the gamma-secretase complex, which also includes presenilin (PSEN1) and nicastrin (NCSTN). The gamma-secretase complex is required for the intramembrane proteolysis of a number of membrane proteins, including the amyloid-beta precursor protein (APP) and Notch. PSENEN is required for Notch pathway signaling, and for the activity and accumulation of gamma-secretase. Mutations resulting in haploinsufficiency for this gene cause familial acne inversa-2 (ACNINV2).