PSD95-Specific Recombinant monoclonal antibody, PBS Only

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  WB analysis using 81106-1-RR (same clone as 81106-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81106-1-RR (PSD95-Specific,DLG4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81106-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 81106-1-RR (same clone as 81106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81106-1-RR (PSD95-Specific,DLG4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81106-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 81106-1-RR (same clone as 81106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81106-1-RR (PSD95-Specific,DLG4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81106-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 81106-1-RR (same clone as 81106-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PSD95-Specific,DLG4 antibody (81106-1-RR, Clone: 1I22 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81106-1-PBS in a different storage buffer formulation.

PSD95-Specific Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 81106-1-PBS

Clone No.1I22

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

PSD95-Specific,DLG4, DLG4, PSD-95, psd95/dlg4, 1I22

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Product Information

81106-1-PBS targets PSD95-Specific in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  fusion protein

• Full Name: discs, large homolog 4 (Drosophila)
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 90-95 kDa
• GenBank Accession Number: NM_001365
• Gene Symbol: PSD95
• Gene ID (NCBI): 1742
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78352
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (disks large homolog 4) gene. PSD-95 is a scaffolding protein of the MAGUK protein family, and engages in several vital protein-protein interactions in the brain with its PDZ domains. It has been suggested that PSD-95 is composed of two supramodules, one of which is the PDZ1-2 tandem domain. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation. Observed MW of PSD95 is 90-95 kDa due to phosphorylation (PMID: 20682303).