PSAT1 Monoclonal antibody, PBS Only

PSAT1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B2C9

Cat no : 67619-1-PBS

Synonyms

EPIP, Phosphoserine aminotransferase, PSA, PSAT, PSAT1

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Validation Data Gallery

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  WB analysis using 67619-1-Ig (same clone as 67619-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67619-1-Ig (PSAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67619-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67619-1-Ig (same clone as 67619-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67619-1-Ig (PSAT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67619-1-PBS in a different storage buffer formulation.

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  IF Staining of HCT 116 using 67619-1-Ig (same clone as 67619-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using PSAT1 antibody (67619-1-Ig, Clone: 1B2C9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67619-1-PBS in a different storage buffer formulation.

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Product Information

67619-1-PBS targets PSAT1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSAT1 fusion protein Ag14076
• Full Name: phosphoserine aminotransferase 1
• Calculated Molecular Weight: 370 aa, 40 kDa
• Observed Molecular Weight: 37-40 kDa
• GenBank Accession Number: BC001618
• Gene Symbol: PSAT1
• Gene ID (NCBI): 29968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PSAT1, also named as PSA and PSAT, belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family and SerC subfamily. It catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. PSAT1 represents a new interesting target for CRC therapy. (PMID:18221502) It may be implicated in altered serine metabolism and schizophrenia spectrum conditions. (PMID:20955740 )